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KMID : 0363320180390030302
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Journal of Korean Oriental Internal Medicine
2018 Volume.39 No. 3 p.302 ~ p.312
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Effects of Agastachis Herba Extract and Lysimachiae Herba Extract on the Experimental Cellular Model of NFLDs Induced by Palmitic Acid
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Lee Hye-In
Kim Young-Kwang
Lim Hyun-Chan
Lee Da-Eun
Kim Eun-Ji
Moon Young-Ho
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Abstract
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Objectives: This study was performed to investigate the effects of two herbal medicines, Agastachis Herba and Lysimachiae Herba, on a cellular model of non-alcoholic fatty liver diseases (NFLDs).
Methods: HepG2 cells were treated with palmitic acid and with various concentrations of Agastachis Herba (AH) or Lysimachiae Herba (LH) extract in water. The lipotoxicity was assessed using EZ-cytox, and the lipoapoptosis was assessed using cell death detection ELISA. Intracellular lipids were measured by oil red O staining. The efficacy of AH and LH on sterol regulatory element-binding transcription factor-1c (SREBP-1c), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) in HepG2 cells was measured by reverse transcription polymerase chain reaction (RT-PCR).
Results: Both AH and LH extracts increased lipoapoptosis and decreased lipotoxicity and levels of SREBP-1c, ACC, and FAS (SREBP-1c, ACC, and FAS are factors in lipid synthesis). In the oil red O staining experiment, both extracts also reduced intracellular lipid accumulation; in this instance, LH’s efficacy was superior to that of AH.
Conclusions: According to the results, both AH and LH are likely to contribute to non-alcoholic fatty liver disease, as both interfere with lipid synthesis.
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KEYWORD
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Agastachis Herba, Lysimachiae Herba, NAFLD
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